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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant, herbicide-tolerant, high yield eucalyptus
EN
H421 × 955S019 × 1521K059
Yes
FGN-Ø1A21-5 × FGN-Ø3S19-5 × FGN-Ø6K59-4
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,Organization:FuturaGene Brasil Tecnologia Ltda ()Private sector (business and industry)
, BrazilPhone: + 55 15 3511 8032,Fax:Email: info@futuragene.com,Website: -
Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/,
The hybrid eucalyptus was modified for resistance to insects, tolerance to herbicides and greater biomass. For resistance to Lepidoptera, the modified eucalyptus expresses three Bacillus thuringiensis crystal proteins Cry1Ab, Cry1Bb and Cry2Aa, which have a pore forming mode-of-action in the epithelial lining of feeding larvae. For tolerance to glyphosate, the modified eucalyptus expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicide with high affinity and thus allows for the continued functioning of the shikimate pathway and biosynthesis of aromatic amino acids and compounds. For greater yield and increased growth rate, the eucalyptus overexpresses Arabidopsis thaliana endo-β‐1,4‐d-glucanase, which remodels the xyloglucan-cellulose matrix of the cell wall during development to promote cell expansion and growth. In addition to these cassettes, the modified eucalyptus contains three Escherichia coli neomycin phosphotransferase II cassettes, which functioned as kanamycin selectable markers during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-272520-2 Organism Eucalyptus grandis × Eucalyptus urophylla (Hybrid eucalyptus)Trees
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BCH-ORGA-SCBD-272517-3 Organism Eucalyptus urophylla (Timor mountain gum, Timor white gum, Eucalyptus, EUCUR)Trees
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BCH-LMO-SCBD-109049-3 Living Modified Organism FGN-Ø1A21-5 - Eucalyptus modified for increased growth and yieldFuturaGene Brasil Tecnologia Ltda | Changes in physiology and/or production (Yield), Changes in quality and/or metabolite content (Cellulose), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-272464-2 Living Modified Organism FGN-Ø3S19-5 - Herbicide-tolerant eucalyptusFuturaGene Brasil Tecnologia Ltda, Suzano S.A. | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to herbicides (Glyphosate), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-272537-1 Living Modified Organism FGN-Ø6K59-4 - Insect-resistant eucalyptusSuzano S.A., FuturaGene Brasil Tecnologia Ltda | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to diseases and pests (Insects, Diptera (flies), Hessian fly (Mayetiola destructor), Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda)), Selectable marker genes and reporter genes
EN
pBI121
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-109045-1 Strawberry vein banding virus promoter | Strawberry vein banding virus (SVBV)Promoter
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BCH-GENE-SCBD-109046-1 Endo‐1,4‐β‐d‐glucanase coding sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Yield)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-272524-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain terminator | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Terminator
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BCH-GENE-SCBD-272521-2 Cry1Bb | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272523-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Promoter
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BCH-GENE-SCBD-115762-1 Leader sequence | Tobacco etch virus (TEV)Leader sequence
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BCH-GENE-SCBD-272522-1 Cry2Aa | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Diptera (flies), Hessian fly (Mayetiola destructor), Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272525-1 Elongation factor EF-1 alpha | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Intron
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
DNA insert from H421 (FGN-Ø1A21-5)
The parental eucalyptus contains two gene cassettes: Arabidopsis thaliana endo-β‐1,4‐d-glucanase (cel1) and Escherichia coli neomycin phosphotransferase II (nptII).
The cel1 sequence is under control of a Strawberry vein banding virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the viral promoter, which induces strong constitutive transcription.
The nptII sequence is under control of a nopaline synthase promoter and terminator.
Note:
The cel1 sequence is under control of a Strawberry vein banding virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the viral promoter, which induces strong constitutive transcription.
The nptII sequence is under control of a nopaline synthase promoter and terminator.
Note:
- Molecular analysis (Southern blot, sequencing and PCR) showed that a single T-DNA insertion occurred into the eucalyptus genome.
- Sequencing of event H421 confirmed that constructs present in the vector were preserved in the transformation process (without rearrangement of the gene cassettes or truncation of the inserted T-DNA sequence).
DNA insert from 955S019 (FGN-Ø3S19-5)
The parental eucalyptus contains three gene cassettes: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes and one Escherichia coli neomycin phosphotransferase II (nptII) cassette.
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
- Molecular characterization indicated that a single T-DNA insertion occurred in the parental eucalyptus genome (in chromosome 3) without inclusion of vector backbone sequences. The sequences are present in the same arrangement as the transformation vector (i.e., without rearrangement). Protein expression was confirmed with ELISA.
DNA insert from 1521K059 (FGN-Ø6K59-4)
The parental eucalyptus contains four gene cassettes: Bacillus thuringiensis crystal protein Cry1Ab, B. thuringiensis crystal protein Cry1Bb, Escherichia coli neomycin phosphotransferase II (nptII) and B. thuringiensis crystal protein Cry2Aa.
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
- Next generation sequencing analyses indicated that parental eucalyptus genome contains a singular T-DNA insertion in chromosome 3 (located at approximately 66 Mbp) without integration of vector backbone sequences. The analyses also indicated that the four cassettes were present as expected (without rearrangements).
- ELISA analyses confirmed expression of Cry1Ab, Cry1Bb, Cry2Aa and NPTII in leaves (young and mature), stems, roots and pollen.
For more information, kindly refer to the parental LMO records.
EN
- Timber
- Other (Paper pulp)
EN
EN
